Easyroti 250 ml (phytoplankton gel for zooplankton culture) EASYREEFS

Easyroti 250 ml - EASYREEFS

Description

Easyroti, for the cultivation of rotifers.

Two species of microalgae, easy reefs® Nannochloropsis (80%) + Tetraselmis (20%), are used for the cultivation of rotifers.

The dose depends on the density of rotifers reached.

The average consumption is 4 ml of Easyroti per liter of rotifer culture per day (25% harvest of rotifers per day).

Available in two formats: 250 ml, 1500 ml, and 3 L

Usage

Necessary equipment: 3 cylindrical containers of 2 L with gentle aeration, using a hollow rod. Initial inoculum of rotifers.

Food needed Easyroti: Nannochloropsis (80%) and Tetraselmis (20%).

Cultivation cycle from the stump: 5 days.

To filter the rotifer, it is necessary to use a mesh of 50 microns.

It consists of a parallel culture system in which, from day 6, a continuous supply for the aquarium is obtained. One pot for "Stock" and another pot for "Production".

Rotifer culture

The detailed dosage data below are under ideal conditions, assuming an ideal rotifer density. Sometimes, the amount of algae added may not meet the rotifer's demand. In case of microalgae depletion (the water remains clear and yellowish), it will indicate that the dose was insufficient. When the rotifer lacks food for more than 4 hours, the number of eggs decreases, and consequently, the population growth rate also declines. It is essential that the rotifer always has food available.

The rotifer is very sensitive to changes in the physical and chemical parameters of the water. If the water quality deteriorates rapidly, the rotifer population will suffer. In the case of excessive dirt in the tube, filter the rotifer and inoculate it into a clean tube and add the necessary algae.

With experience, the intensity of the green color will indicate the amount of algae you need, and you might even develop your own protocol. There is no difference between using live algae and Easyroti as food.

A rod with a diameter of 5 mm is introduced into the culture tube with very gentle aeration (5-10 bubbles per second) to ensure a homogeneous mixture of the culture. Excessive turbulence causes the rotifer to lose its eggs and they do not hatch.

It is recommended to distribute the feeding dose into two or more doses per day, but due to time constraints, the rotifer is usually fed once a day.

DAY 1

Initial conditions: Culture "Strain" with 500 ml. Number of rotifers 400 rot./ml to 600 rot./ml and 4 ml of Easyroti Nannochloropsis (80%) + Tetraselmis (20%).

Final conditions: The culture will have a final volume of 1 L with algae.

User manual: Disperse 4 ml of Easyroti in 0.5 L of seawater. Add the rotifer inoculum.

DAY 2

Initial conditions: 1 L of rotifer culture. It will be almost transparent and almost without algae. Estimated number of rotifers: 500 rot/ml.

Final conditions: The culture will have a final volume of 1.4 L with algae.

User manual: Disperse 4 ml of Easyroti in 0.5 L of seawater and add 0.4 L to the rotifer culture.

DAY 3

Initial conditions: 1.4 L of rotifer culture. It will be almost transparent and almost without algae. Estimated number of rotifers: 500-600 rot/ml.

Final conditions: The culture will have a final volume of 1.8 L with algae.

User manual: Disperse 8 ml of Easyroti in 0.5 L of seawater and add 0.4 L to the rotifer culture.

DAY 4

Initial conditions: 1.8 L of rotifer culture. It will be almost transparent and almost without algae. Estimated number of rotifers: 600 rot/ml

Final conditions: The culture will have a final volume of 2 L with algae.

User manual: Disperse 6 ml of Easyroti in 0.25 L of seawater and add 0.2 L to the rotifer culture.

DAY 5

Initial conditions: 2 L of rotifer culture. It will be almost transparent and almost without algae. Estimated number of rotifers: 700-800 rot/ml

From this "Cepa" culture, a partial harvest of 0.5 L is conducted to start a new "Cepa" culture in another 2 L pot and begin a new cycle as on day 1.

The former "Cepa" crop will now be the "Production" crop. We will add Easyroti and increase it to 2 L. If the pot is too dirty, we need to transfer it to another clean one to continue the production crop.

Instructions: In the "Production" culture, we will add 8 ml of Easyroti previously dispersed in 0.5 L of seawater. In the "Cepa" culture, start a new cycle from the first day.

DAY 6

Initial conditions: Culture "Strain" as on day 2. The "Production" culture 2 L at 800 rot/ml will be almost transparent and nearly without algae.

The "Production" culture will be used to harvest 0.5 L per day to be used as food in the aquarium (if the aquarium is not supplied, the harvest must also be done to feed the rotifer with algae). Each time 0.5 L is harvested, 0.5 L must be added with 8 ml of Easyroti.

The "production" culture will be kept until the pot is dirty (5-7 days).

When the "Cepa" culture reaches its 5th day, it will become a "Production" culture and a new "Cepa" culture will be created in parallel, as explained for the 5th day.

We will empty the old "Production" culture and proceed with its cleaning and disinfection.